Rapid cell extraction in aqueous two-phase microdroplet systems

Rapid One-Step Separation and Purification of Recombinant Phenylalanine Dehydrogenase in Aqueous Two-Phase Systems

Background: Phenylalanine dehydrogenase (PheDH EC 1.4.1.20) is a NAD+-dependent enzyme that performs the reversible oxidative deamination of L-phenylalanine to phenylpyruvate. It plays an important role in detection and screening of phenylketonuria (PKU) diseases and production of chiral intermediates as well. The main goal of this study was to find a simple and rapid alternative method for pur...

Effect of Junction Geometry on Monodispersed Microdroplet Generation in Microfluidic Aqueous Two-Phase Systems

Extraction of Cephalexin Using Aqueous Two-Phase Systems Composed of Cholinium Chloride and K3PO4

Cephalexin is an important antibiotic. It is very significant to determine an appropriate method for the extraction of this valuable antibiotic for industrial applications. Aiming at developing an efficient method for the extraction of cephalexin, the partitioning of cephalexin has been evaluated in aqueous two-phase system, including cholinium chloride and potassium phosphate. The effect of th...

Aqueous Two-Phase Systems

ger G and Albertsson PA (1983) AfRnity partitioning of phosphofructokinase from baker’s yeast using polymer-bound Cibacron blue F3GA. European Journal of Biochemistry 131: 589}594. KopperschlaK ger G and Birkenmeier G (1990) AfRnity partitioning and extraction of proteins. Bioseparation 1: 235}254. Tjerneld F, Johansson G and Joelsson M (1987) AfRnity liquid}liquid extraction of lactate dehydro...

rapid one-step separation and purification of recombinant phenylalanine dehydrogenase in aqueous two-phase systems

background: phenylalanine dehydrogenase (phedh ec 1.4.1.20) is a nad+-dependent enzyme that performs the reversible oxidative deamination of l-phenylalanine to phenylpyruvate. it plays an important role in detection and screening of phenylketonuria (pku) diseases and production of chiral intermediates as well. the main goal of this study was to find a simple and rapid alternative method for pur...